Number of the records: 1
Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus
- 1.
SYSNO ASEP 0351320 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Transcription-dependent rearrangements of actin and nuclear myosin I in the nucleolus Author(s) Philimonenko, Vlada (UMG-J)
Janáček, Jiří (FGU-C) RID, ORCID
Harata, M. (JP)
Hozák, Pavel (UMG-J) RID, ORCIDSource Title Histochemistry and Cell Biology. - : Springer - ISSN 0948-6143
Roč. 134, č. 3 (2010), s. 243-249Number of pages 7 s. Language eng - English Country DE - Germany Keywords RNA-polymerase-I ; ultrastructural analysis ; UBF Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/07/1592 GA ČR - Czech Science Foundation (CSF) 2B06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ME09101 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50110509 - FGU-C (2005-2011) UT WOS 000281249500002 DOI 10.1007/s00418-010-0732-8 Annotation Nuclear actin and nuclear myosin I (NMI) are important players in transcription of ribosomal genes. Transcription of rDNA takes place in highly organized intranuclear compartment, the nucleolus. In this study, we characterized the localization of these two proteins within the nucleolus of HeLa cells with high structural resolution by means of electron microscopy and gold-immunolabeling. We demonstrate that both actin and NMI are localized in specific compartments within the nucleolus, and the distribution of NMI is transcription-dependent. Moreover, a pool of NMI is present in the foci containing nascent rRNA transcripts. Actin, in turn, is present both in transcriptionally active and inactive regions of the nucleolus and colocalizes with RNA polymerase I and UBF. Our data support the involvement of actin and NMI in rDNA transcription and point out to other functions of these proteins in the nucleolus, such as rRNA maturation and maintenance of nucleolar architecture. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
Number of the records: 1