Number of the records: 1

Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata

SYSNO ASEP	0351175
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Functional molecular analysis of a circadian clock gene timeless promoter from the drosophilid fly Chymomyza costata
Author(s)	Kobelková, Alena (BC-A) Bajgar, Adam (BC-A)_RID Doležel, David (BC-A)_{RID, ORCID}
Number of authors	3
Source Title	Journal of Biological Rhythms. - : Sage - ISSN 0748-7304 Roč. 25, č. 6 (2010), s. 399-409
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	timeless promoter ; E-box ; Chymomyza costata
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GP204/08/P579 GA ČR - Czech Science Foundation (CSF)
	GA204/07/1032 GA ČR - Czech Science Foundation (CSF)
	IAA500960802 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000284302400002
DOI	10.1177/0748730410385283
Annotation	The tim promoter from D. melanogaster has been studied in detail both in tissue cultures and in living flies yet has never been investigated in other species. This article presents a detailed functional analysis of the tim promoter from the drosophilid fly, Chymomyza costata, in Drosophila tissue cultures. A comparison of tim promoters from wt and npd-mutants confirmed that the 1855 bp deletion in the latter removes crucial regulatory cis-elements as well as the minimal promoter, being subsequently responsible for the lack of tim mRNA expression. Deletion and substitution mutations of the wt tim promoter showed that the region containing the canonical E-box, TER-box, and 2 incomplete E-box sequences is essential for CLK/CYC-mediated expression, while the PERR element appears to be a repressor in S2 cells. Furthermore, the expression of the circadian genes timeless, period, vrille, and doubletime was quantified in C. costata adults.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1