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Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus

SYSNO ASEP	0351168
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus
Author(s)	Sklenář, Jan (MBU-M) Niku-Paavola, M. - L. (FI) Santos, S. (PT) Man, Petr (MBU-M)_{RID, ORCID} Kruus, K. (FI) Novotný, Čeněk (MBU-M)_{RID, ORCID}
Source Title	Enzyme and Microbial Technology. - : Elsevier - ISSN 0141-0229 Roč. 46, č. 7 (2010), s. 550-556
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	Solid-support growth ; White-rot fungus ; Irpex lacteus
Subject RIV	EE - Microbiology, Virology
R&D Projects	IAAX00200901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GP310/08/P258 GA ČR - Czech Science Foundation (CSF)
	LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000278612700002
DOI	10.1016/j.enzmictec.2010.03.001
Annotation	This paper desribes optimalization of the growth conditions of white-rot fungus Irpex lacteus, a potential candidate for use in bioremediation in situ, on polyurethane solid support. The main objective was isolation and characterization of manganese-dependent peroxidase (MnP) isoenzyme (pI 4.8) exhibitng the highest activity. Optimization of the enzyme production and isolation procedure covering anion exchange chromatography (Mono Q) was performed. The purified protein was subjected to LC-MSMS characterization which provided information on the amino acid sequence as well as the glycosylation
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1