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The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation

SYSNO ASEP	0351017
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and Cell Plate Maturation
Author(s)	Fendrych, Matyáš (UEB-Q) Synek, Lukáš (UEB-Q)_{RID, ORCID} Pečenková, Tamara (UEB-Q)_{RID, ORCID} Toupalová, Hana (UEB-Q) Cole, R. (US) Drdová, Edita (UEB-Q)_{RID, ORCID} Nebesářová, Jana (BC-A)_{RID, ORCID} Šedinová, M. (CZ) Hála, Michal (UEB-Q)_{RID, ORCID} Fowler, J.E. (US) Žárský, Viktor (UEB-Q)_{RID, ORCID}
Source Title	Plant Cell. - : Oxford University Press - ISSN 1040-4651 Roč. 22, č. 9 (2010), s. 3053-3065
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	ELECTRON TOMOGRAPHIC ANALYSIS ; SACCHAROMYCES-CEREVISIAE ; PLANT TRANSFORMATION
Subject RIV	ED - Physiology
R&D Projects	LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KJB600380806 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	KJB600380802 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000283579800013
DOI	10.1105/tpc.110.074351
Annotation	Secretory pathways play a crucial role in cytokinesis in plants from the onset of phragmoplast initiation to the maturation of the cell plate. Tethering of secretory vesicles at the plasma membrane is mediated by the exocyst complex. Using proteomic and cytologic approaches, we show that EXO84b is a subunit of the plant exocyst. A. thaliana mutants for EXO84b are severely dwarfed and have compromised leaf epidermal cell and guard cell division. During cytokinesis, green fluorescent protein– tagged exocyst subunits SEC6, SEC8, SEC15b, EXO70A1, and EXO84b exhibit distinctive localization maxima at cell plate initiation and cell plate maturation, stages with a high demand for vesicle fusion. Finally, we present data indicating a defect in cell plate assembly in the exo70A1 mutant. We conclude that the exocyst complex is involved in secretory processes during cytokinesis in Arabidopsis cells, notably in cell plate initiation, cell plate maturation, and formation of new primary cell wall.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1