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Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard
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SYSNO ASEP 0351013 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Isolation and characterization of two new lipopeptide biosurfactants produced by Pseudomonas fluorescens BD5 isolated from water from the Arctic Archipelago of Svalbard Author(s) Janek, T. (PL)
Lukaszewicz, M. (PL)
Řezanka, Tomáš (MBU-M) ORCID
Krasowska, A. (PL)Source Title Bioresource Technology. - : Elsevier - ISSN 0960-8524
Roč. 101, č. 15 (2010), s. 6118-6123Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords Pseudomonas fluorescens BD5 ; Lipopeptide ; Pseudofactins Subject RIV EE - Microbiology, Virology CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000278035900052 DOI 10.1016/j.biortech.2010.02.109 Annotation The arctic freshwater bacterium Pseudomonas fluorescens BD5 produces biosurfactants when grown on 2 percent glucose. Crude biosurfactants were extracted from a cell-free culture supernatant with ethyl acetate and purified by preparative reversed phase high performance liquid chromatography (RP-HPLC). The chemical structure of the purified biosurfactants, pseudofactin I and II, was analyzed by matrix assisted laser desorption/ionization time of flight (MALDI TOF) mass spectrometry and tandem mass spectrometry (MS/MS). Both compounds are novel cyclic lipopeptides with a palmitic acid connected to the terminal amino group of eighth amino acid in peptide moiety Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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