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Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI
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SYSNO ASEP 0351011 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Pilot-plant cultivation of Streptomyces griseus producing homologues of nonactin by precursor-directed biosynthesis and their identification by LC/MS-ESI Author(s) Řezanka, Tomáš (MBU-M) ORCID
Prell, Aleš (MBU-M)
Spížek, Jaroslav (MBU-M)
Sigler, Karel (MBU-M) RIDSource Title Journal of Antibiotics. - : Springer - ISSN 0021-8820
Roč. 63, č. 8 (2010), s. 524-529Number of pages 6 s. Language eng - English Country JP - Japan Keywords liquid chromatography/MS-ESI ; pilot-plant cultivation ; precursor-directed biosynthesis Subject RIV EE - Microbiology, Virology R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281499800018 DOI 10.1038/ja.2010.93 Annotation Precursor-directed biogenetic approach was used to produce a range of nonactin homologues in a 50 l fermentor cultivation of strain Streptomyces griseus 34/249 obtained by UV mutagenesis. The production medium contained sodium propionate, isobutyrate and isovalerate as individual precursors, and 10 g l/1 Diaion HP20 styrene-divinylbenzene resin that maintains suitable precursor concentration by reversibly adsorbing and releasing it. The produced nonactin homologues were separated on two C18 reversed-phase liquid chromatography columns in series and analyzed by MS with ESI source in the positive ion mode. Formation of doubly charged ions was suppressed by an excess of Na ions throughout the process. The production of the homologues increased up to day 5 and then it leveled off. Cultivations with individual precursors yielded a total of 18 nonactin homologues whose spectrum depended on the precursor used Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
Number of the records: 1