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Enzymatic Kinetic Resolution of Silybin Diastereoisomers

SYSNO ASEP	0350993
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Enzymatic Kinetic Resolution of Silybin Diastereoisomers
Author(s)	Monti, D. (IT) Gažák, Radek (MBU-M)_{RID, ORCID} Marhol, Petr (MBU-M)_RID Biedermann, David (MBU-M)_{RID, ORCID} Purchartová, Kateřina (MBU-M)_RID Fedrigo, M. (IT) Riva, S. (IT) Křen, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Journal of Natural Products. - : American Chemical Society - ISSN 0163-3864 Roč. 73, č. 4 (2010), s. 613-619
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	MARIANUM MILK THISTLE ; PROSTATE-CANCER ; SILYMARIN
Subject RIV	CC - Organic Chemistry
R&D Projects	GAP207/10/0288 GA ČR - Czech Science Foundation (CSF)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	OC08049 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000276950000019
DOI	10.1021/np900758d
Annotation	In nature, the flavonolignan silybin (1) occurs as a mixture of two diastereomers, silybin A and silybin B, which in a number of biological assays exhibit different activities. A library of hydrolases (lipases, esterases, and proteases) was tested for separating the silybin A and B diastereomers by selective transcsterification or by stereoselective alcoholysis of 23-O-acetylsilybin (2). Novozym 435 proved to be the most suitable enzyme for the preparative production of both optically pure silybins A and B by enzymatic discrimination. Gram amounts of the optically pure substances can be produced within one week, and the new method is robust and readily scalable to tens of grams
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1