Number of the records: 1

Reduction of heteroduplex formation in PCR amplification

SYSNO ASEP	0350890
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Reduction of heteroduplex formation in PCR amplification
Author(s)	Michu, Elleni (BFU-R) Mráčková, Martina (BFU-R) Vyskot, Boris (BFU-R)_{RID, ORCID} Žlůvová, Jitka (BFU-R)_{RID, ORCID}
Number of authors	4
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 54, č. 1 (2010), s. 173-176
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	polymerase chain reaction ; reconditioning PCR ; mixed-template PCR
Subject RIV	BO - Biophysics
R&D Projects	KJB600040801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GD204/09/H002 GA ČR - Czech Science Foundation (CSF)
	IAA600040801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000276699500029
Annotation	We detected that the heteroduplex to homoduplex ratio can be decreased by the addition of Taq polymerase and by a decrease in the number of PCR cycles.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2011

Number of the records: 1