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Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo
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SYSNO ASEP 0350885 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Nedbal, Ladislav (UEK-B)
Tříska, Jan (UEK-B) RID, ORCID, SAI
Kotas, Petr (UEK-B) RID
Trtílek, M. (CZ)Number of authors 8 Source Title Sensors. - : MDPI
Roč. 10, č. 9 (2010), s. 8635-8651Number of pages 17 s. Language eng - English Country CH - Switzerland Keywords Raman spectroscopy ; algal cells ; lipids ; iodine value Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) GA206/09/1284 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z20650511 - UPT-D (2005-2011) AV0Z60870520 - UEK-B (2005-2011) UT WOS 000282218800045 DOI 10.3390/s100908635 Annotation lgae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. This potential has stimulated the development of innovative analytical methods focused on these microorganisms. Algal lipids are among the most promising potential products for fuels as well as for nutrition. The crucial parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids quantified by the iodine value. Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. The Raman spectra were collected from three selected algal species immobilized in an agarose gel. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2011
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