Number of the records: 1

New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses

SYSNO ASEP	0350494
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses
Author(s)	Špak, Josef (BC-A)_{RID, ORCID} Holý, Antonín (UOCHB-X) Pavingerová, Daniela (BC-A) Votruba, Ivan (UOCHB-X)_RID Špaková, Vlastimila (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Antiviral Research. - : Elsevier - ISSN 0166-3542 Roč. 88, č. 3 (2010), s. 296-303
Number of pages	8 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Brassica ; Chemotherapy ; Turnip yellow mosaic virus ; Ribavirin
Subject RIV	CE - Biochemistry
R&D Projects	GA522/09/0707 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000285660300008
DOI	10.1016/j.antiviral.2010.09.019
Annotation	A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1