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Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants

  1. 1.
    SYSNO ASEP0350379
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCharacterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants
    Author(s) Dwivedi, S. (US)
    Vaňková, Radomíra (UEB-Q) RID, ORCID
    Motyka, Václav (UEB-Q) RID, ORCID
    Herrera, C. (US)
    Žižková, Eva (UEB-Q) ORCID
    Auer, C. (US)
    Source TitlePlant Growth Regulation. - : Springer - ISSN 0167-6903
    Roč. 61, č. 3 (2010), s. 231-242
    Number of pages12 s.
    Languageeng - English
    CountryNL - Netherlands
    KeywordsCytokinin metabolism ; N-Glucosyltransferase ; GRAM domain
    Subject RIVED - Physiology
    R&D ProjectsIAA600380701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    ME 505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    CEZAV0Z50380511 - UEB-Q (2005-2011)
    UT WOS000278411500003
    DOI10.1007/s10725-010-9467-8
    AnnotationCytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants.
    WorkplaceInstitute of Experimental Botany
    ContactDavid Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
    Year of Publishing2011
Number of the records: 1  

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