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Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants
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SYSNO ASEP 0350379 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants Author(s) Dwivedi, S. (US)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Motyka, Václav (UEB-Q) RID, ORCID
Herrera, C. (US)
Žižková, Eva (UEB-Q) ORCID
Auer, C. (US)Source Title Plant Growth Regulation. - : Springer - ISSN 0167-6903
Roč. 61, č. 3 (2010), s. 231-242Number of pages 12 s. Language eng - English Country NL - Netherlands Keywords Cytokinin metabolism ; N-Glucosyltransferase ; GRAM domain Subject RIV ED - Physiology R&D Projects IAA600380701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) ME 505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000278411500003 DOI 10.1007/s10725-010-9467-8 Annotation Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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