Number of the records: 1
New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss
- 1.
SYSNO ASEP 0350371 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title New data on aetiology of nodular gill disease in rainbow trout, Oncorhynchus mykiss Author(s) Dyková, Iva (BC-A) RID
Kostka, Martin (BC-A) RID
Wortberg, F. (DE)
Nardy, E. (DE)
Pecková, Hana (BC-A) RID, ORCIDSource Title Folia Parasitologica. - : Biologické centrum AV ČR, v. v. i. - ISSN 0015-5683
Roč. 57, č. 3 (2010), s. 157-163Number of pages 7 s. Language eng - English Country CZ - Czech Republic Keywords nodular gill disease ; aetiological study ; amoebae ; Naegleria sp. ; fish diseases ; aquaculture ; Oncorhynchus mykiss Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine R&D Projects GA524/09/0137 GA ČR - Czech Science Foundation (CSF) LC522 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000282543400001 DOI 10.14411/fp.2010.021 Annotation We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus on kiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (I), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperolastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1