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Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo
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SYSNO ASEP 0350109 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo Author(s) Borghese, L. (DE)
Doležalová, Dáša (UEM-P)
Opitz, T. (DE)
Haupt, S. (DE)
Leinhaas, A. (DE)
Steinfarz, B. (DE)
Koch, P. (DE)
Edenhofer, F. (DE)
Hampl, Aleš (UEM-P)
Brüstle, O. (DE)Source Title Stem Cells. - : Oxford University Press - ISSN 1066-5099
Roč. 28, č. 5 (2010), s. 955-964Number of pages 10 s. Language eng - English Country US - United States Keywords neural stem cells ; notch ; neuron Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50390703 - UEM-P (2007-2013) UT WOS 000278497400012 DOI 10.1002/stem.408 Annotation The controlled in vitro differentiation of human embryonic stem cells (hESCs) and other pluripotent stem cells provides interesting prospects for generating large numbers of human neurons for a variety of biomedical applications. A major bottleneck associated with this approach is the long time required for hESC-derived neural cells (hESNSCs) to give rise to functional neuronal progeny. Here we assessed the expression of Notch pathway components in hESNSCs and demonstrate that Notch signaling is active under self-renewing culture conditions. Combined with growth factor withdrawal, inhibition of Notch signaling results in a marked acceleration of differentiation, thereby shortening the time required for the generation of electrophysiologically active hESNSC-derived neurons. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2011
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