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Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition
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SYSNO ASEP 0349941 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Infrared spectroscopy-based metabolomic analysis of maize growing under different nitrogen nutrition Author(s) Pavlík, Milan (UEB-Q) RID, ORCID
Pavlíková, D. (CZ)
Vašíčková, Soňa (UOCHB-X)Source Title Plant, Soil and Environment. - : Česká akademie zemědělských věd - ISSN 1214-1178
Roč. 56, č. 11 (2010), s. 533-540Number of pages 8 Language eng - English Country CZ - Czech Republic Keywords ammonium ; ecdysone phosphate ; CULTAN Subject RIV GD - Fertilization, Irrigation, Soil Processing R&D Projects 1G58027 GA MZe - Ministry of Agriculture (MZe) 2B06024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000284283600005 Annotation For metabolomic analysis of maize plants growing under different nitrogen nutrition sequential extraction of fresh biomass was used and isolated fractions were characterized and evaluated using IR spectra. The IR spectra of individual fractions were evaluated in relation to the nitrogen rates (2 g or 4 g N), to applied fertilizers (ammonium nitrogen or urea ammonium nitrate solution) and sampling period. For butanol fraction, typical bands of flavonoids, polar phospholipids, steryl glycosides, analogues of ecdysteroids were characterized. The IR spectra of BuOH fraction showed changes of relative contents of isolated compounds mainly affected by nitrogen rates. For water fraction bands of organic acids, salts of organic acids, flavonoid glycosides and oligopeptides (phytochelatins and/or glutathione) were the most significant. The results showed an increased induction of oxalic acid in plants after 4 g N application. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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