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Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana
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SYSNO ASEP 0349937 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana Author(s) Wimalasekera, R. (DE)
Pejchar, Přemysl (UEB-Q) RID, ORCID
Holk, A. (DE)
Martinec, Jan (UEB-Q) RID, ORCID
Scherer, G.F.E. (DE)Source Title Molecular Plant. - : Cell Press - ISSN 1674-2052
Roč. 3, č. 3 (2010), s. 610-625Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords Auxin ; brassinolide signaling ; phosphate deficiency Subject RIV ED - Physiology R&D Projects LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA522/07/1614 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000278819900013 DOI 10.1093/mp/ssq005 Annotation Phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphocholine and diacylglycerol (DAG). PC-PLC has a long tradition in animal signal transduction to generate DAG as a second messenger besides the classical phosphatidylinositol splitting phospholipase C (PI-PLC). Based on amino acid sequence similarity to bacterial PC-PLC, six putative PC-PLC genes (NPC1 to NPC6) were identified in the Arabidopsis genome. RT-PCR analysis revealed overlapping expression pattern of NPC genes in root, stem, leaf, flower, and silique. In auxin-treated P-NPC3:GUS and P-NPC4:GUS seedlings, strong increase of GUS activity was visible in roots, leaves, and shoots and, to a weaker extent, in brassinolide-treated (BL) seedlings. P-NPC4:GUS seedlings also responded to cytokinin with increased GUS activity in young leaves. Compared to wild-type, T-DNA insertional knockouts npc3 and npc4 showed shorter primary roots and lower lateral root density. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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