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Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting
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SYSNO ASEP 0349793 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Evaluation of the denaturing gradient gel electrophoresis-apparatus as a parameter influencing soil microbial community fingerprinting Author(s) Ascher, J. (IT)
Ceccherini, M.T. (IT)
Chroňáková, Alica (BC-A) RID, ORCID
Jirout, Jiří (BC-A) RID
Borgogni, F. (IT)
Elhottová, Dana (BC-A) RID, ORCID
Šimek, Miloslav (BC-A) RID, ORCID
Pietramellara, G. (IT)Source Title World Journal of Microbiology and Biotechnology. - : Springer - ISSN 0959-3993
Roč. 26, č. 9 (2010), s. 1721-1726Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords cattle impact ; DGGE ; DGGE interfering factors Subject RIV EH - Ecology, Behaviour R&D Projects LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA600660605 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000280642800021 DOI 10.1007/s11274-010-0349-z Annotation We compared two denaturing gradient gel electrophoresis (DGGE) systems-DCode (Biorad, Hercules, CA, USA) and PhorU (Ingeny, Leiden, NL), performing community level 16S and 18S rRNA gene fragment-PCR-DGGE with total DNA extracted from upland pasture soil used for outdoor cattle husbandry. The methodological evaluation of the DGGE apparatus as parameter influencing DGGE fingerprinting, based on cluster analysis of soil bacterial and fungal community fingerprints, was made in terms of the resulting information about microbial community structures and their response to different degrees of cattle impact. Although the comparative DGGE analysis with different DGGE systems provided similar clustering of microbial community structures in correlation with the degree of cattle impact, our results suggest the DGGE system to be a factor influencing DGGE analysis. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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