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Fiber-optic pH detection in small volumes of biosamples
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SYSNO ASEP 0349751 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fiber-optic pH detection in small volumes of biosamples Author(s) Kašík, Ivan (URE-Y) RID
Mrázek, Jan (URE-Y) RID, ORCID
Martan, Tomáš (URE-Y)
Pospíšilová, Marie (URE-Y)
Podrazký, Ondřej (URE-Y) RID
Matějec, Vlastimil (URE-Y)
Hoyerová, Klára (UEB-Q) RID, ORCID
Kamínek, Miroslav (UEB-Q) RID, ORCIDNumber of authors 8 Source Title Analytical and Bioanalytical Chemistry. - : Springer - ISSN 1618-2642
Roč. 398, č. 5 (2010), s. 1883-1889Number of pages 7 s. Language eng - English Country DE - Germany Keywords optical fiber sensor ; plants ; pH Subject RIV JB - Sensors, Measurment, Regulation R&D Projects LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GP102/08/P639 GA ČR - Czech Science Foundation (CSF) IAA600380805 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z20670512 - URE-Y (2005-2011) AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000283244500007 DOI 10.1007/s00216-010-4130-9 Annotation The paper deals with the fiber-optic detection of the pH values of droplets of plant xylem exudate based on fluorescence intensity measurements. For this purpose, novel V-taper sensing probes with a minimum diameter of around 8 μm were prepared that enable the delivery of fluorescence signal from the detection site on the taper tip to the detector. The taper tips were coated with pH-sensitive HPTS and a reference Ru-phen dichloride immobilized in a xerogel layer. Using the developed V-taper sensing probes, the pH values of in vivo and in vitro samples of small volumes (6 μl) of exudate were measured. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2011
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