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Differences in cold adaptation of .i.Bacillus subtilis./i. under anaerobic and aerobic conditions
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SYSNO ASEP 0349691 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions Author(s) Beranová, J. (CZ)
Mansilla, M.C. (AR)
de Mendoza, D. (AR)
Elhottová, Dana (BC-A) RID, ORCID
Konopásek, I. (CZ)Source Title Journal of Bacteriology. - : American Society for Microbiology - ISSN 0021-9193
Roč. 192, č. 16 (2010), s. 4164-4171Number of pages 8 s. Language eng - English Country US - United States Keywords cold adaptation ; Bacillus subtilis ; anaerobiosis Subject RIV EE - Microbiology, Virology R&D Projects LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000280406300010 DOI 10.1128/JB.00384-10 Annotation Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the temperature downshift and is decreased when membrane fluidity is restored. B. subtilis is also capable of anaerobic growth by nitrate or nitrite respiration. We studied the mechanism of cold adaptation in B. subtilis under anaerobic conditions that were predicted to inhibit Des activity. We found that in anaerobiosis, in contrast to aerobic growth, the induction of des expression after temperature downshift (from 37°C to 25°C) was not downregulated. However, the transfer from anaerobic to aerobic conditions rapidly restored the downregulation. However, the transfer from anaerobic to aerobic conditions rapidly restored the downregulation. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1