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Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process
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SYSNO ASEP 0349615 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Utilization of the linear mode of MALDI-TOF mass spectrometry in the study of glycation during the malting process Author(s) Laštovičková, Markéta (UIACH-O) RID, ORCID
Mazanec, Karel (UIACH-O)
Smětalová, Dagmar (UIACH-O)
Bobálová, Janette (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Journal of the Institute of Brewing. - : Wiley - ISSN 0046-9750
Roč. 116, č. 3 (2010), s. 245-250Number of pages 4 s. Language eng - English Country GB - United Kingdom Keywords barley ; brewing ; glycation Subject RIV CB - Analytical Chemistry, Separation R&D Projects 1M0570 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40310501 - UIACH-O (2005-2011) UT WOS 000287278700005 Annotation The analysis of intact proteins, extracted from the barley grain, the malt and the aliquots collected during malting and presented in this paper, show the feasibility of using the linear mode of MALDI-TOF MS to obtain a profile of the proteins present at all stages of the malting process. The best and the most reproducible results were obtained after a desalting procedure using Nanosep 3K and applying DHAP as a matrix for the MS measurement of the intact proteins. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2011
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