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Immobilization of histidine-tagged proteins on monodisperse metallochelation liposomes: Preparation and study of their structure
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SYSNO ASEP 0349452 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Immobilization of histidine-tagged proteins on monodisperse metallochelation liposomes: Preparation and study of their structure Author(s) Mašek, J. (CZ)
Bartheldyová, E. (CZ)
Korvasová, Z. (CZ)
Škrabalová, J. (CZ)
Koudelka, Š. (CZ)
Kulich, P. (CZ)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAI
Miller, A. D. (GB)
Ledvina, Miroslav (UOCHB-X) RID
Raška, M. (CZ)
Turánek, J. (CZ)Source Title Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 408, č. 1 (2011), s. 95-104Number of pages 10 s. Language eng - English Country US - United States Keywords liposome ; proteoliposome ; detergent removal method ; recombinant protein ; metallochelatation ; AF microscopy ; TEM ; dynamic light scattering Subject RIV CE - Biochemistry R&D Projects GAP304/10/1951 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z10100520 - FZU-D (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000283886600013 DOI 10.1016/j.ab.2010.08.023 Annotation Liposomes represent a biocompatible platform for the construction of self-assembling proteoliposomes using nickel- or zinc-metallochelation. Here, we describe the design and construction of a flow-through ultrafiltration cell suitable for the preparation of monodisperse liposomes enabled for metallochelation and hence the formation of proteoliposomes. These model proteoliposomes are characterised by gel permeation chromatography and by dynamic light scattering. Transmission electron microscopy and immunogold staining are used to characterize surface bound proteins, revealing the tendency of rgp120 to form microdomains on liposome surfaces. These microdomains possess a 2D crystal-like structure that is seen more precisely by atomic force (AF) microscopy. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2011
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