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The Noncanonical Gag Domains p8 and n Are Critical for Assembly and Release of Mouse Mammary Tumor Virus

SYSNO ASEP	0349410
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Noncanonical Gag Domains p8 and n Are Critical for Assembly and Release of Mouse Mammary Tumor Virus
Author(s)	Zábranský, Aleš (UOCHB-X)_{RID, ORCID} Hoboth, Peter (UOCHB-X) Hadravová, Romana (UOCHB-X)_{RID, ORCID} Štokrová, Jitka (UOCHB-X) Sakalian, M. (US) Pichová, Iva (UOCHB-X)_{RID, ORCID}
Number of authors	6
Source Title	Journal of Virology - ISSN 0022-538X Roč. 84, č. 21 (2010), s. 11555-11559
Number of pages	5 s.
Language	eng - English
Country	US - United States
Keywords	betaretrovirus ; Gag ; assembly
Subject RIV	CE - Biochemistry
R&D Projects	1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000282643400059
DOI	10.1128/JVI.00652-10
Annotation	The mouse mammary tumor virus (MMTV) Gag contains the unique domains pp21, p3, p8 and n. We investigated the contribution of these domains to particle assembly and found that the region spanning the p8 and n domains is critical for shape determination and assembly. Deletion of pp21 and p3 reduced the number of released particles, but deletion of the n domain resulted in frequent formation of aberrant particles, while deletion of p8 severely impaired assembly. Further investigation of the p8 revealed that both the basic and the proline-rich motifs within p8 contribute to MMTV assembly.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1