Number of the records: 1

Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

SYSNO ASEP	0349181
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat
Author(s)	Šafář, Jan (UEB-Q)_{RID, ORCID} Šimková, Hana (UEB-Q)_{RID, ORCID} Kubaláková, Marie (UEB-Q)_RID Čihalíková, Jarmila (UEB-Q)_RID Suchánková, Pavla (UEB-Q)_RID Bartoš, Jan (UEB-Q)_{RID, ORCID} Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Cytogenetic and Genome Research. - : Karger - ISSN 1424-8581 Roč. 129, 1-3 (2010), s. 211-223
Number of pages	13 s.
Language	eng - English
Country	CH - Switzerland
Keywords	BAC library ; Chromosome ; DNA markers
Subject RIV	GE - Plant Breeding
R&D Projects	GA521/07/1573 GA ČR - Czech Science Foundation (CSF)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000280519500023
DOI	10.1159/000313072
Annotation	The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1