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Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement

SYSNO ASEP	0349037
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement
Author(s)	Humpolíčková, Jana (UFCH-W)_RID Benda, Aleš (UFCH-W)_{RID, ORCID} Macháň, Radek (UFCH-W) Enderlein, J. (DE) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076 Roč. 12, č. 39 (2010), s. 12457-12465
Number of pages	9 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	fluorescence microscopy ; dunamic saturation optical microscopy ; fluorescence
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KJB400400904 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
UT WOS	000282643900013
DOI	10.1039/c0cp00059k
Annotation	Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2011

Number of the records: 1