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Magnetic affinity separation of recombinant fusion proteins

SYSNO ASEP	0348877
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Magnetic affinity separation of recombinant fusion proteins
Author(s)	Šafařík, Ivo (UEK-B)_{RID, SAI} Šafaříková, Miroslava (UEK-B)_{RID, SAI}
Number of authors	2
Source Title	Hacettepe Journal of Biology and Chemistry - ISSN 1303-5002 Roč. 38, č. 1 (2010), s. 1-7
Number of pages	7 s.
Language	eng - English
Country	TR - Turkey
Keywords	recombinant fusion proteins ; affinity tags ; magnetic separation
Subject RIV	CE - Biochemistry
R&D Projects	OC 157 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	2A-1TP1/094 GA MPO - Ministry of Industry and Trade (MPO)
CEZ	AV0Z60870520 - UEK-B (2005-2011)
Annotation	Isolation and separation of proteins is an important procedure in all areas of biosciences and biotechnology. Currently recombinant fusion proteins are produced routinely; in order to simplify their isolation from complex samples, magnetic separation techniques can be efficiently used. This short review paper shows examples of the most important fusion affinity tags and commercially available magnetic affinity and ion exchange particles enabling efficient capture of recombinant fusion proteins. A list of realized isolation procedures documents the efficiency of magnetic separation techniques.
Workplace	Global Change Research Institute
Contact	Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268
Year of Publishing	2011

Number of the records: 1