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Membrane Active Peptides. Methods and Results on Structure and Function

SYSNO ASEP	0348380
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Peptide-Membrane Interactions Studied by Ellipsometry, Laser Scanning Microscopy, and Z-Scan Fluorescence Correlation Spectroscopy
Author(s)	Miszta, Adam (UFCH-W) Macháň, Radek (UFCH-W) Hermens, W. Th. (NL) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Membrane Active Peptides. Methods and Results on Structure and Function. - La Jolla : International University Line, 2010 / Castanho M. A. R. B. - ISBN 978-0-9720774-5-3
Pages	s. 219-245
Number of pages	27 s.
Number of pages	635
Language	eng - English
Country	US - United States
Keywords	ellipsometry ; laser scanning microscopy ; fluorescence microscopy
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40400503 - UFCH-W (2005-2011)
DOI	10.3890/978-0-9720774-5-3
Annotation	The following chapter presents two optical experimental techniques: confocal fluorescence microscopy (CFM) and ellipsometry, and focuses on their applications in studying interactions of antimicrobial peptides with supported phospholipid bilayers (SPBs). The principal properties of SPBs are mentioned together with their use as geometrically well-defined models of biological membranes. The basics of each of techniques are explained and their possibilities and limitations are discussed. Images with diffraction-limited resolution are obtained by CFM showing the spatial distribution of fluorescent molecules in the sample. Selective labeling offers the possibility to visualize location of different molecular species. Furthermore, the signal from each point of the sample can be analyzed separately; the measurement of molecular mobility in individual points of the sample by fluorescence correlation spectroscopy is addressed in more detail.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2011

Number of the records: 1