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RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis
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SYSNO ASEP 0348166 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RP-HPLC/MS-APCI Analysis of Branched Chain TAG Prepared by Precursor-Directed Biosynthesis with Rhodococcus erythropolis Author(s) Schreiberová, O. (CZ)
Krulikovská, T. (CZ)
Sigler, Karel (MBU-M) RID
Čejková, A. (CZ)
Řezanka, Tomáš (MBU-M) ORCIDSource Title Lipids. - : Wiley - ISSN 0024-4201
Roč. 45, č. 8 (2010), s. 743-756Number of pages 14 s. Language eng - English Country US - United States Keywords Rhodococcus erythropolis ; RP-HPLC/MS-APCI ; Branched chain triacylglycerols Subject RIV EE - Microbiology, Virology R&D Projects 2B08062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000281027800009 DOI 10.1007/s11745-010-3447-7 Annotation Reversed phase liquid chromatography–atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1–3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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