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Biochemical and Genetic Insights into Asukamycin Biosynthesis
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SYSNO ASEP 0348161 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biochemical and Genetic Insights into Asukamycin Biosynthesis Author(s) Rui, Z. (US)
Petříčková, Kateřina (MBU-M) RID
Škanta, František (MBU-M) RID
Pospíšil, Stanislav (MBU-M) RID
Yang, Y. (US)
Chen, Ch.-Y. (TW)
Tsai, S.-F. (TW)
Floss, H. G. (US)
Petříček, Miroslav (MBU-M) RID
Yu, T.-W. (TW)Source Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 285, č. 32 (2010), s. 24915-24924Number of pages 10 s. Language eng - English Country US - United States Keywords NODOSUS SUBSP ASUKAENSIS ; FATTY-ACID SYNTHASE ; CRYSTAL-STRUCTURE Subject RIV EE - Microbiology, Virology R&D Projects 2B06154 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000280542100064 DOI 10.1074/jbc.M110.128850 Annotation Asukamycin, a member of the manumycin family metabolites, is an antimicrobial and potential antitumor agent isolated from Streptomyces nodosus subsp. asukaensis. The entire asukamycin biosynthetic gene cluster was cloned, assembled, and expressed heterologously in Streptomyces lividans. Bioinformatic analysis and mutagenesis studies elucidated the biosynthetic pathway at the genetic and biochemical level. Four gene sets, asuA–D, govern the formation and assembly of the asukamycin building blocks: a 3-amino-4-hydroxybenzoic acid core component, a cyclohexane ring, two triene polyketide chains, and a 2-amino-3-hydroxycyclopent-2-enone moiety to form the intermediate protoasukamycin Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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