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Large-scale separation of silybin diastereoisomers using lipases

SYSNO ASEP	0348130
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Large-scale separation of silybin diastereoisomers using lipases
Author(s)	Gažák, Radek (MBU-M)_{RID, ORCID} Marhol, Petr (MBU-M)_RID Purchartová, Kateřina (MBU-M)_RID Monti, D. (IT) Biedermann, David (MBU-M)_{RID, ORCID} Riva, S. (IT) Cvak, L. (CZ) Křen, Vladimír (MBU-M)_{RID, ORCID}
Source Title	Process Biochemistry. - : Elsevier - ISSN 1359-5113 Roč. 45, č. 10 (2010), s. 1657-1663
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Silybin A ; silymarin ; silibinin
Subject RIV	CC - Organic Chemistry
R&D Projects	GA303/08/0658 GA ČR - Czech Science Foundation (CSF)
	LC06010 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP207/10/0288 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000282388800009
DOI	10.1016/j.procbio.2010.06.019
Annotation	The flavonolignan silybin (1), isolated from the seeds of milk thistle (Silybum marianum), occurs in nature as an equimolar mixture of two diastereoisomers, silybin A and silybin B, that exhibit different biological activities. The preparative production of optically pure silybin A and B in a diastereoisomeric purity greater than 95 percent was accomplished using immobilized Candida antarctica lipase B (Novozym 435) in a combination of two reactions: regioselective acetylation of a natural silybin mixture (1) and subsequent stereoselective alcoholysis of the resulting 23-O-acetylsilybin (2). Several grams of the optically pure substances can be produced within one week using this new, robust and scalable process, which is selective, mild and high-yielding
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1