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Inflammasome Activation by Adenylate Cyclase Toxin Directs Th17 Responses and Protection against Bordetella pertussis

SYSNO ASEP	0348119
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Inflammasome Activation by Adenylate Cyclase Toxin Directs Th17 Responses and Protection against Bordetella pertussis
Author(s)	Dunne, A. (IE) Ross, P. J. (IE) Pospíšilová, Eva (MBU-M) Mašín, Jiří (MBU-M)_{RID, ORCID} Meaney, A. (IE) Sutton, C. E. (IE) Iwakura, Y. (JP) Tschopp, J. (CH) Šebo, Peter (MBU-M)_{RID, ORCID} Mills, K. H. G. (IE)
Source Title	Journal of Immunology. - : American Association of Immunologists - ISSN 0022-1767 Roč. 187, č. 3 (2010), s. 1711-1719
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	ADAPTIVE IMMUNE-RESPONSES ; IL-17-PRODUCING T-CELLS ; HOST-DEFENSE
Subject RIV	EC - Immunology
R&D Projects	GA310/08/0447 GA ČR - Czech Science Foundation (CSF)
	IAA500200914 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50200510 - MBU-M (2005-2011)
UT WOS	000280177400048
DOI	10.4049/jimmunol.1000105
Annotation	In this article, we demonstrate that inflammasome-mediated IL-1b plays a critical role in promoting Ag-specific Th17 cells and in generating protective immunity against Bordetella pertussis infection. Using a murine respiratory challenge model, we demonstrated that the course of B. pertussis infection was significantly exacerbated in IL-1R type I-defective (IL-1RI2/2) mice. We found that adenylate cyclase toxin (CyaA), a key virulence factor secreted by B. pertussis, induced robust IL-1b production by dendritic cells through activation of caspase-1 and the NALP3-containing inflammasome complex. Using mutant toxins, we demonstrate that CyaA-mediated activation of caspase-1 was not dependent on adenylate cyclase enzyme activity but was dependent on the pore-forming capacity of CyaA
Workplace	Institute of Microbiology
Contact	Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
Year of Publishing	2011

Number of the records: 1