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2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing
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SYSNO ASEP 0348081 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing Author(s) Petry-Podgórska, Inga (UIACH-O)
Žídková, Jitka (UIACH-O)
Flodrová, Dana (UIACH-O)
Bobálová, Janette (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 878, č. 30 (2010), s. 3143-3148Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords 2D-HPLC ; MALDI-TOF/TOF mass spectrometry ; barley Subject RIV CB - Analytical Chemistry, Separation R&D Projects 1M0570 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40310501 - UIACH-O (2005-2011) UT WOS 000284672300014 DOI 10.1016/j.jchromb.2010.09.023 Annotation A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun” approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2011
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