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Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava

SYSNO ASEP	0348004
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava
Author(s)	Zhang, P. (CH) Wang, W.Q. (CN) Zhang, G. L. (CN) Kamínek, Miroslav (UEB-Q)_{RID, ORCID} Dobrev, Petre (UEB-Q)_{RID, ORCID}
Source Title	Journal of Integrative Plant Biology - ISSN 1672-9072 Roč. 52, č. 7 (2010), s. 653-669
Number of pages	17 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	MANIHOT-ESCULENTA CRANTZ ; TRANSGENIC CASSAVA ; AGROBACTERIUM-TUMEFACIENS
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA600380805 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000279167200005
DOI	10.1111/j.1744-7909.2010.00956.x
Annotation	Cassava (Manihot esculenta Crantz) sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-IPT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1