Number of the records: 1

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

SYSNO ASEP	0347925
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection
Author(s)	Takasu, Yoko (BC-A) Kobayashi, I. (JP) Beumer, K. (US) Uchino, K. (JP) Sezutsu, H. (JP) Sajwan, S. (CZ) Carroll, D. (US) Tamura, T. (JP) Žurovec, Michal (BC-A)_{RID, ORCID}
Number of authors	9
Source Title	Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748 Roč. 40, č. 10 (2010), s. 759-765
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	gene targeting ; nonhomologous and joining ; Lepidoptera
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	IAA500070601 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP305/10/2406 GA ČR - Czech Science Foundation (CSF)
	LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000283390300007
DOI	10.1016/j.ibmb.2010.07.012
Annotation	The absence of a general gene-targeting system for systematic reverse genetic studies has been a significant limitation in silkworm research. The authors performed direct zinc finger nuclease mRNA injection to /B. mori/ embryos and induced mutations in an epidermal color gene /BmBLOS2. /The results suggest that the /B. mori/ double-strand break repair system relies on microhomologies rather than on a ligase IV-dependent mechanism typical for /Drosophila/. The observed frequency of germline mutations was sufficient for future use in gene targeting relying solely on molecular methods of screening.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1