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Autocrine effects of visfatin on hepatocyte sensitivity to insulin action
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SYSNO ASEP 0347884 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Autocrine effects of visfatin on hepatocyte sensitivity to insulin action Author(s) Škop, V. (CZ)
Kontrová, K. (CZ)
Zídek, Václav (FGU-C) RID
Pravenec, Michal (FGU-C) RID, ORCID
Kazdová, L. (CZ)
Mikulík, Karel (MBU-M)
Sajdok, J. (CZ)
Zídková, J. (CZ)Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 59, č. 4 (2010), s. 615-618Number of pages 3 s. Language eng - English Country CZ - Czech Republic Keywords Visfatin ; RNA interference ; insulin mimetic effects Subject RIV FB - Endocrinology, Diabetology, Metabolism, Nutrition R&D Projects IAA500110805 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) ME08006 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NR9387 GA MZd - Ministry of Health (MZ) NR9359 GA MZd - Ministry of Health (MZ) CEZ AV0Z50110509 - FGU-C (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000282054700016 Annotation Visfatin was originally described as an adipokine with insulin mimetic effects. Since liver plays a major role in glucose metabolism, we studied visfatin effects on insulin-stimulated cellular glucose uptake in Fao rat hepatocytes using RNA interference (RNAi). RNAi-mediated downregulation of visfatin expression in Fao cells was associated with significantly reduced NAD biosynthesis (0.3+/-0.01 vs. 0.5+/-0.01 mmol/h/g, P<0.05) and with significantly decreased incremental glucose uptake after stimulation with insulin when compared to controls with normal expression of visfatin (0.6+/-0.2 vs. 2.2+/-0.5 nnmol/g/2 h, P=0.02). These results provide evidence that visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2011
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