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Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy

SYSNO ASEP	0347391
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Size determination of Acipenser ruthenus spermatozoa in different types of electron microscopy
Author(s)	Pšenička, M. (CZ) Tesařová, Martina (BC-A)_ORCID Těšitel, J. (CZ) Nebesářová, Jana (BC-A)_{RID, ORCID}
Source Title	Micron. - : Elsevier - ISSN 0968-4328 Roč. 41, č. 5 (2010), s. 455-460
Number of pages	6 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	Scanning electron microscopy ; Size determination ; Sterlet spermatozoa ; Sterlet spermatozoa
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000278790400010
DOI	10.1016/j.micron.2010.02.004
Annotation	In this study three types of scanning electron microscopes were used for the size determination of spermatozoa of sterlet Acipenser ruthenus - high vacuum scanning electron microscope (SEM, JEOL 6300), environmental scanning electron microscope (ESEM, Quanta 200 FEG), field emission scanning electron microscope (FESEM, JEOL 7401F) with cryoattachment Alto 2500 (Gatan) and transmission electron microscope (TEM, JEOL 1010). The use of particular microscopes was tied with different specimen preparation techniques. The aim of this study was to evaluate to what degree the type of used electron microscope can influence the size of different parts of spermatozoa. Results confirmed that the classical chemical procedure of specimen preparation for SEM including critical point drying method led to a significant contraction of all measured values, which could deviate up to 30% in comparison with values measured on the fresh chemically untreated specimen in ESEM.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1