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The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei

SYSNO ASEP	0347296
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The Fe/S Cluster Assembly Protein Isd11 Is Essential for tRNA Thiolation in Trypanosoma brucei
Author(s)	Paris, Zdeněk (BC-A)_RID Changmai, Piya (BC-A) RUBIO, M. A. T. (US) Zíková, Alena (BC-A)_{RID, ORCID} Stuart, K. D. (US) Alfonzo, J. D. (US) Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258 Roč. 285, č. 29 (2010), s. 22394-22402
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	IRON-SULFUR PROTEINS ; SACCHAROMYCES-CEREVISIAE ; CYSTEINE DESULFURASE ; THIO-MODIFICATION ; FRATAXIN
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1667 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000279702200052
DOI	10.1074/jbc.M109.083774
Annotation	Fe/S clusters are part of the active site of many enzymes and are essential for cell viability. In eukaryotes the cysteine desulfurase Nfs (IscS) donates the sulfur during Fe/S cluster assembly and was thought sufficient for this reaction. Moreover, Nfs is indispensable for tRNA thiolation, a modification generally required for tRNA function and protein synthesis. Recently, Isd11 was discovered as an integral part of the Nfs activity at an early step of Fe/S cluster assembly. Here we show, using a combination of genetic, molecular, and biochemical approaches, that Isd11, in line with its strong association with Nfs, is localized in the mitochondrion of T. brucei. In addition to its involvement in Fe/S assembly, Isd11 also partakes in both cytoplasmic and mitochondrial tRNA thiolation, whereas Mtu1, another protein proposed to collaborate with Nfs in tRNA thiolation, is required for this process solely within the mitochondrion.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1