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YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei

SYSNO ASEP	0347288
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei
Author(s)	Týč, Jiří (BC-A)_{ORCID, RID} Long, Shaojun (BC-A) Jirků, Milan (BC-A)_RID Lukeš, Julius (BC-A)_{RID, ORCID}
Source Title	Molecular and Biochemical Parasitology. - : Elsevier - ISSN 0166-6851 Roč. 173, č. 1 (2010), s. 43-47
Number of pages	5 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Trypanosoma ; Plastid ; Mitochondrion ; Targeting ; YCF45 ; Horizontal gene transfer
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1667 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000279706900007
DOI	10.1016/j.molbiopara.2010.05.002
Annotation	YCF45 belongs to a family of proteins of unknown function usually located in the chloroplast of plants. Its highly conserved homologues were found in the genomes of several Trypanosoma and Leishmania species. HA(3)-tagging of the YCF45 protein with the start codon as annotated in the Gene(DB) revealed its cytosolic localization in the cultured procyclic stage of Trypanosoma brucei. However, when a more upstream located start codon was used in another HA(3)-tagged construct, the resulting protein was targeted to the mitochondrion. We propose that YCF45 was acquired by an ancestral trypanosomatid by horizontal gene transfer and in the absence of a plastid was re-targeted to the mitochondrion.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2011

Number of the records: 1