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Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation
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SYSNO ASEP 0347281 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Benzimidazole resistance allele haplotype diversity in United Kingdom isolates of Teladorsagia circumcincta supports a hypothesis of multiple origins of resistance by recurrent mutation Author(s) Skuce, P. (GB)
Stenhouse, L. (GB)
Jackson, F. (GB)
Hypša, Václav (BC-A) RID
Gilleard, J. (CA)Source Title International Journal for Parasitology. - : Elsevier - ISSN 0020-7519
Roč. 40, č. 11 (2010), s. 1247-1255Number of pages 9 s. Language eng - English Country AU - Australia Keywords BZ resistance ; b-Tubulin ; Haplotype diversity ; Haplotype diversity Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000280936100003 DOI 10.1016/j.ijpara.2010.03.016 Annotation Polymorphisms in the isotype 1 beta-tubulin gene are important genetic determinants of benzimidazole (BZ) resistance in a number of parasitic nematode species including Teladorsagia circumcincta, a major gastrointestinal nematode of sheep. This study investigates the genetic diversity at this locus in a BZ-resistant Isolate of T circumcincta (MTc15) derived from a sheep farm in UK that was open to animal, and therefore parasite, migration. Pyrosequencing was used to determine the frequency of single nucleotide polymorphisms (SNPs) known to be associated with BZ resistance. This was followed by a combination of single strand conformation polymorphism (SSCP) analysis and nucleotide sequencing to sample allelic diversity in a 276 bp fragment immediately surrounding the isotype I beta-tubulin F200Y mutation The genetic diversity at this locus was extremely high, with seven different haplotypes found to contain the resistant F200Y polymorphism in this single resistant isolate. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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