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Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point
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SYSNO ASEP 0347130 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Terminal restriction fragment length measurement errors are affected mainly by fragment length, G+C nucleotide content and secondary structure melting point Author(s) Bukovská, Petra (BU-J)
Jelínková, Markéta (MBU-M)
Hršelová, Hana (MBU-M) RID, ORCID
Sýkorová, Zuzana (BU-J) RID
Gryndler, Milan (MBU-M) RID, ORCIDNumber of authors 5 Source Title Journal of Microbiological Methods. - : Elsevier - ISSN 0167-7012
Roč. 82, č. 3 (2010), s. 223-228Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords T-RFLP ; drift ; DNA fragment Subject RIV EF - Botanics R&D Projects 1M0571 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z60050516 - BU-J (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000282113000005 DOI 10.1016/j.mimet.2010.06.007 Annotation Several methods of molecular analysis of microbial diversity, including terminal restriction fragment length polymorphism (T-RFLP) analysis are based on measurement of the DNA fragment length. Significant variation between sequence-determined and measured length of restriction fragments (drift) has been observed, which can affect the efficiency of the identification of microorganisms in the analyzed communities. In this study, principal component analysis and multiple regression analysis revealed that secondary structure melting point and G+C nucleotide content, besides the fragment length, contribute to the variation observed, whereas the contribution of purine Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2011
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