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Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation
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SYSNO ASEP 0347082 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of Carbonic Anhydrase I Immunodominant Epitopes Recognized by Specific Autoantibodies Which Indicate an Improved Prognosis in Patients with Malignancy after Autologous Stem Cell Transplantation Author(s) Skultety, L. (SK)
Jankovičová, B. (SK)
Svobodová, Z. (CZ)
Mader, Pavel (UMG-J)
Řezáčová, Pavlína (UMG-J) RID
Dubrovčáková, M. (SK)
Lakota, J. (SK)
Bílková, Z. (SK)Source Title Journal of Proteome Research. - : American Chemical Society - ISSN 1535-3893
Roč. 9, č. 10 (2010), s. 5171-5179Number of pages 9 s. Language eng - English Country US - United States Keywords epitope mapping ; carbonic anhydrase I ; spontaneous remission Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA203/09/0820 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000282257800027 DOI 10.1021/pr1004778 Annotation Spontaneous tumor regression was observed in patients who developed aplastic anaemia type syndrome after high-dose therapy with autologous stem cell transplantation and whose sera contained high titer of anti-carbonic anhydrase (anti-CA) autoantibodies. Many indications suggest that presence of these autoantibodies provides significant survival benefit for the patients. Western blot analysis confirmed immunoreactivity of the patients’ sera with several CA isoforms. Highly abundant and widely distributed CA I was selected for epitope mapping study. The applied analytical approach consisted of specific fragmentation of CA I followed by immunospecific isolation of peptides reacting with polyclonal anti-CA I autoantibodies. Standard epitope mapping schema was improved by incorporating magnetic carriers and biomagnetic separation techniques. Mass spectrometry was used for detection and identification of epitopes and acquired results were verified by bioinformatic tools. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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