Number of the records: 1

Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images

SYSNO ASEP	0347023
Document Type	C - Proceedings Paper (int. conf.)
R&D Document Type	Conference Paper
Title	Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images
Author(s)	Michálek, Jan (FGU-C)_RID Čapek, Martin (FGU-C)_{RID, ORCID} Mao, X. W. (US) Kubínová, Lucie (FGU-C)_{RID, ORCID}
Source Title	Analysis of biomedical signals and images. - Brno : University of technology, 2010 - ISSN 1211-412X - ISBN 978-80-214-4106-4
Pages	s. 49-54
Number of pages	6 s.
Action	International EURASIP Conference Biosignal /20./
Event date	27.06.2010-29.06.2010
VEvent location	Brno
Country	CZ - Czech Republic
Event type	WRD
Language	eng - English
Country	CZ - Czech Republic
Keywords	CLSM ; illumination inhomogeneities
Subject RIV	JD - Computer Applications, Robotics
R&D Projects	GA102/08/0691 GA ČR - Czech Science Foundation (CSF)
	GA304/09/0733 GA ČR - Czech Science Foundation (CSF)
	LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	MEB090910 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000303723700008
Annotation	Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain, is presented. The method does not require any special calibration (reference) images since the gain estimate is extracted from the CLSM image being corrected itself. The proposed approach exploits two types of morphological filters: the median filter and a morphological operator called the upper Lipschitz cover
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2011

Number of the records: 1