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Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization
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SYSNO ASEP 0346865 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization Author(s) Yi, Y.J. (KR)
Manandhar, G. (US)
Sutovky, M. (US)
Zimmerman, S.W. (US)
Jonáková, Věra (BTO-N) RID
Leeuwen van, F.W. (NL)
Oko, R. (CA)
Park, Ch.S. (KR)
Sutovsky, P. (US)Number of authors 9 Source Title Cell and Tissue Research - ISSN 0302-766X
Roč. 341, č. 2 (2010), s. 325-340Number of pages 16 s. Language eng - English Country DE - Germany Keywords Sperm ; Proteasome ; Fertilization ; Zona pellucida ; Acrosome ; Alzheimer's ; Porcine Subject RIV CE - Biochemistry R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA303/09/1285 GA ČR - Czech Science Foundation (CSF) NS10009 GA MZd - Ministry of Health (MZ) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000280593600010 Annotation Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for mammalian fertilization. The study examined the role of PSMD4, a subunit of 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane. In conclusion, the 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for porcine/mammalian fertilization in vitro. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2011
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