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A lens-specific co-injection marker for medaka transgenesis
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SYSNO ASEP 0346841 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A lens-specific co-injection marker for medaka transgenesis Author(s) Vopálenský, Pavel (UMG-J)
Růžičková, Jana (UMG-J)
Pavlů, Barbora (UMG-J)
Kozmik, Zbyněk (UMG-J) RIDSource Title Biotechniques - ISSN 0736-6205
Roč. 48, č. 3 (2010), s. 235-236Number of pages 2 s. Language eng - English Country US - United States Keywords lens ; transgenic ; crystallin Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA500520908 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 1M0520 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000277562300011 DOI 10.2144/000113368 Annotation Recent advances in fish transgenesis allow generation of transgenic animals at high numbers and efficiency of germ-line transmission. To facilitate the procedure, a suitable co-injection marker may be very helpful for preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well defined tissue positioned at the periphery and is easily accessible for examination of reporter gene expression. Here, we constructed reporter vectors based on mouse gamma-F crystallin (mF-Cry) promoter driving heterologous expression of the reporter gene at a high level and very specifically to the lens allowing an easy sorting of transgenics. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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