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Ribonucleoprotein localization in mouse oocytes
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SYSNO ASEP 0346833 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ribonucleoprotein localization in mouse oocytes Author(s) Flemr, Matyáš (UMG-J)
Svoboda, Petr (UMG-J) RIDSource Title Methods. - : Elsevier - ISSN 1046-2023
Roč. 53, č. 2 (2011), s. 136-141Number of pages 6 s. Language eng - English Country US - United States Keywords mouse oocyte ; in situ hybridization ; immunofluorescence Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/0085 GA ČR - Czech Science Foundation (CSF) GAP305/10/2215 GA ČR - Czech Science Foundation (CSF) ME09039 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000287275900004 DOI 10.1016/j.ymeth.2010.08.005 Annotation RNA molecules rarely function alone in cells. For most RNAs, their function requires formation of various ribonucleoprotein (RNP) complexes. For example, mRNP composition can determine mRNA localization, translational repression, level of translation or mRNA stability. RNPs are usually studied by biochemical methods. However, biochemical approaches are unsuitable for some model systems, such as mammalian oocytes and early embryos, due to the small amounts that can be obtained for experimental analysis. In such cases, microscopic techniques are often used to learn about RNPs. Here, we present a review of immunostaining, fluorescence in situ hybridization with subcellular resolution and a combination of both, with emphasis on the mouse oocyte and early embryos models. Application of these techniques to whole-mount fixed oocytes and early embryos can provide information about RNP composition and localization with three-dimensional resolution. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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