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Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells

SYSNO ASEP	0346830
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells
Author(s)	Blume, Y. (UA) Yemets, A. (UA) Sheremet, Y. (UA) Nyporko, A. (UA) Sulimenko, Vadym (UMG-J)_{RID, ORCID} Sulimenko, Tetyana (UMG-J) Dráber, Pavel (UMG-J)_{RID, ORCID}
Source Title	BMC Plant Biology. - : BioMed Central - ISSN 1471-2229 Roč. 10, x (2010), s. 29-29
Number of pages	1 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	beta-tubulin ; plant microtubule ; epitope
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000275663600002
DOI	10.1186/1471-2229-10-29
Annotation	This research article reports on the correlation between localization of five small beta-tubulin regions on A. thaliana microtubule protofilament model and their exposure on cortical microtubules in cells. Using homology modeling it was generated the model for the prediction of surface exposure beta-tubulin epitopes and tyrosine residues. Peptide scans disclosed the position of epitopes and following verification of the results was done by immunofluorescence microscopy concerning that beta-tubulins could be one of the targets for tyrosine kinases. This article can contribute to a better understanding of the interactions of plant microtubules with associated proteins.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2011

Number of the records: 1