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Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts
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SYSNO ASEP 0346787 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Down-regulation of protein-tyrosine phosphatases activates an immune receptor in the absence of its translocation into lipid rafts Author(s) Heneberg, Petr (UMG-J)
Dráberová, Lubica (UMG-J) RID
Bambousková, Monika (UMG-J)
Pompach, Petr (MBU-M) RID, ORCID
Dráber, Petr (UMG-J) RIDSource Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 285, č. 17 (2010), s. 12787-12802Number of pages 16 s. Language eng - English Country US - United States Keywords mast cell ; cell signaling ; plasma membrane Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA301/09/1826 GA ČR - Czech Science Foundation (CSF) GAP302/10/1759 GA ČR - Czech Science Foundation (CSF) 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50520514 - UMG-J (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000276787800037 DOI 10.1074/jbc.M109.052555 Annotation In mast cells and basophils activated by multivalent antigen-IgE complexes, tyrosine phosphorylation of the high affinity IgE receptor (FcepsilonRI) is mediated by Src family protein tyrosine (PTK) kinase Lyn. However, the exact molecular mechanism of this phosphorylation step is incompletely understood. In this study, we tested the hypothesis that changes in activity and/or topography of protein-tyrosine phosphatases (PTPs) could play a major role in the FcepsilonRI triggering. We found that exposure of rat basophilic leukemia cells or mouse bone marrow-derived mast cells to PTP inhibitors, H2O2 or pervanadate, induced phosphorylation of the FcepsilonRI subunits, similarly as FcepsilonRI triggering. Interestingly, and in sharp contrast to antigen-induced activation, neither H2O2 nor pervanadate induced any changes in the association of FcepsilonRI with detergent-resistant membranes on isolated plasma membrane sheets. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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