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Method for resolution and quantification of components of the non-photochemical quenching (qN)
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SYSNO ASEP 0346771 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Method for resolution and quantification of components of the non-photochemical quenching (qN) Author(s) Roháček, Karel (BC-A) RID Number of authors 1 Source Title Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 105, č. 2 (2010), s. 101-113Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords Dark relaxation ; Fluorescence quenching ; Multi-exponential regression Subject RIV BO - Biophysics R&D Projects IAA600960716 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000280921200003 DOI 10.1007/s11120-010-9564-6 Annotation A new method of the chlorophyll (Chl) fluorescence quenching analysis is described, which allows the calculation of values of (at least) three components of the non-photochemical quenching of the variable Chl a fluorescence (qN) using a non-linear regression of a multi-exponential function within experimental data. Formulae for coefficients of the “energy”-dependent quenching (qE), the state-transition quenching (qT) and the photo/inhibitory quenching (qI) of Chl a fluorescence were found on the basis of three assumptions: (i) the dark relaxation kinetics of qN, as well as of all its components, is of an exponential nature, (ii) the superposition principle is valid for individual Chl a fluorescence quenching processes, and (iii) the same reference fluorescence level (Fv) is used to define both qN and its components. All definitions as well as the algorithms for analytical recognition of the qN components are theoretically clarified and experimentally tested. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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