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Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas
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SYSNO ASEP 0346752 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Proviruses selected for high and stable expression of transduced genes accumulate in broadly transcribed genome areas Author(s) Plachý, Jiří (UMG-J) RID
Kotáb, Jan (UMG-J)
Divina, Petr (UMG-J)
Reinišová, Markéta (UMG-J)
Šenigl, Filip (UMG-J) RID
Hejnar, Jiří (UMG-J) RIDSource Title Journal of Virology - ISSN 0022-538X
Roč. 84, č. 9 (2010), s. 4204-4211Number of pages 8 s. Language eng - English Country US - United States Keywords avian sarcoma virus ; retrovirus integration ; position effects Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/07/1030 GA ČR - Czech Science Foundation (CSF) GP301/09/P667 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000276358000009 DOI 10.1128/JVI.02511-09 Annotation Retroviruses and retrovirus-derived vectors integrate non-randomly into the genomes of host cells with specific preferences for transcribed genes, gene-rich regions, and CpG islands. To understand the genomic features that influence transcriptinal activity of integrated retroviruses, we cloned and analyzed the integration sites of avian sarcoma virus from chicken tumors. Growing progressively in dependence on high and stable expression of the transduced v-src oncogene, these tumors represent clonal expansions of cells bearing transcriptionally active replication-defective proviruses. Our analysis showed striking accumulation within or close to transcription units, particularly in genes broadly expressed in multiple tissues but not in tissue-specifically expressed genes. We infer that proviruses integrated in these genomic areas efficiently avoid transcriptional silencing and keep active for a long time during the growth of tumors. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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