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Single- and Double-Headed Chemical Probes for Detection of Active Cathepsin D in a Cancer Cell Proteome

SYSNO ASEP	0346346
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Single- and Double-Headed Chemical Probes for Detection of Active Cathepsin D in a Cancer Cell Proteome
Author(s)	Nussbaumerová, Martina (UOCHB-X)_RID Srp, Jaroslav (UOCHB-X)_{RID, ORCID} Máša, Martin (UOCHB-X) Hradilek, Martin (UOCHB-X)_ORCID Šanda, Miloslav (UOCHB-X) Reiniš, Milan (UMG-J)_RID Horn, Martin (UOCHB-X)_{RID, ORCID} Mareš, Michael (UOCHB-X)_{RID, ORCID}
Number of authors	8
Source Title	Chembiochem. - : Wiley - ISSN 1439-4227 Roč. 11, č. 11 (2010), s. 1538-1541
Number of pages	4 s.
Language	eng - English
Country	DE - Germany
Keywords	cathepsin D ; cancer ; activity-based probes
Subject RIV	CE - Biochemistry
R&D Projects	IAA400550705 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000281383200012
DOI	10.1002/cbic.201000182
Annotation	We present the design of activity-based probes for cathepsins D that are effective in a complex proteome. The probes consist of a binding module with a statin-containing peptidic core, a warhead module bearing a photoreactive group, and a reporter tag. The fluorescent and biotin tags make the probes a powerful tool for a wide range of "in-gel" and "on-blot" proteomic applications.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1