Number of the records: 1

Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells

SYSNO ASEP	0346305
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells
Author(s)	Mertlíková-Kaiserová, Helena (UOCHB-X)_{RID, ORCID} Votruba, Ivan (UOCHB-X)_RID Matoušová, Marika (UOCHB-X)_ORCID Holý, Antonín (UOCHB-X) Hájek, Miroslav (UOCHB-X)_{RID, ORCID}
Number of authors	5
Source Title	Anticancer Research. - : International Institute of Anticancer Research - ISSN 0250-7005 Roč. 30, č. 7 (2010), s. 2791-2798
Number of pages	8 s.
Language	eng - English
Country	GR - Greece
Keywords	acyclic nucleoside phosphonate ; programmed cell death ; CCRF-CEM cells ; cell cycle
Subject RIV	CC - Organic Chemistry
R&D Projects	1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000280796400043
Annotation	9-[2-(phosphonomethoxy)ethyl] guanine (PMEG) is a guanine acyclic nucleotide analog whose prodrugs are being investigated for chemotherapy of lymphomas. The objective of this study was to determine the requirements for caspase and CD95/Fas activation in PMEG-induced apoptosis and the influence of PMEG on cell cycle regulatory proteins in CCRF-CEM cells. As for cell cycle regulation, cyclin E1 was found to be up-regulated following PMEG treatment. Although an increase of caspase 3, 8 and 9 proteolytic activity was observed, neither pretreatment of the cells with cell-permeable caspase inhibitors nor blocking the death receptor with anti-Fas antibody did prevent apoptosis induced by PMEG. It was concluded that PMEG-induced apoptosis is caspase- and CD95/Fas-independent.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2011

Number of the records: 1