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Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system
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SYSNO ASEP 0346291 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Conditions for succseful labeling of oxidation sensitive peptides by Na[125-I] - IODO-GEN(TM) system Author(s) Elbert, Tomáš (UOCHB-X) RID, ORCID
Veselá, Iva (UOCHB-X)Number of authors 2 Source Title Journal of Labelled Compounds and Radiopharmaceuticals. - : Wiley - ISSN 0362-4803
Roč. 53, 5/6 (2010), s. 288-291Number of pages 4 s. Action International Symposium on the Synthesis and Applications of Isotopes and Isotopically Labelled Compounds /10./ Event date 14.06.2009-18.06.2009 VEvent location Chicago Country US - United States Event type WRD Language eng - English Country GB - United Kingdom Keywords Iodo-Gen ; peptide ; 125-I ; radiolabelling Subject RIV CC - Organic Chemistry R&D Projects LA 288 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000279381200017 DOI 10.1002/jlcr.1765 Annotation Very good and reproducible yields of 125-I-labeled peptides containing tryptophan residue were achieved when the radioidination reaction was performed with 1 to 3 mCi of carrrier free Na[125-I] in 1.5 ml eppendorf PP tubes precoated with 10 nmols of IODO-GEN(TM) in phosphate buffer saline (pH=7.8). Addition of bovine serum albumin to the solutions of radiolabeled peptides greatly reduced the losses during their workup. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2011
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